<i>Ne2</i> , a typical CC–NBS–LRR‐type gene, is responsible for hybrid necrosis in wheat
نویسندگان
چکیده
Hybrid necrosis, caused by complementary genes Ne1 and Ne2, is a serious barrier for combining desirable traits from different genotypes of wheat, affecting the full utilisation heterosis. To date, both Ne2 are still not isolated although they were documented decades ago. We report here map-based cloning functional characterisation encoding coiled coil–nucleotide-binding site–leucine-rich repeat (CC–NBS–LRR) protein. Homozygous frameshift mutations generated using CRISPR/Cas9 approach confirmed Ne2-inducing hybrid necrosis in wheat. Upregulated expression induced excess hydrogen peroxide accumulation associated with formation. Genetic analyses allele (Ne2m) leaf rust resistance gene LrLC10/Lr13 revealed that might be same gene. Furthermore, we demonstrated frequency was much lower landraces (2.00%) compared modern cultivars (13.62%), suggesting has been partially applied wheat genetic improvement. Our findings open opportunities thoroughly investigating molecular mechanism selecting Lr13 simultaneously avoiding breeding through marker-assisted selection.
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ژورنال
عنوان ژورنال: New Phytologist
سال: 2021
ISSN: ['0028-646X', '1469-8137']
DOI: https://doi.org/10.1111/nph.17575